Experiment A Part III. Preparationof a Plant Extract and Analysis By
Qualitative Tests and TLC
In this part of the experiment, you will walk through the steps required to prepare a plant
extract from raw plant material, then analyze the results of your plant extract being tested in
the qualitative tests discussed in Part I as well as assessing the extract contents by analysis
with thin layer chromatography (TLC, discussed in Part II.)
This procedure will make use of the following techniques. Please follow the links to read
more about these techniques before continuing through the procedure.
Plants are a rich source of unique organic compounds, referred to as “natural products”.
Many drugs (est. 70 to 80%) used in Western medicine originate directly or indirectly from
nature (mostly from plants or microorganisms). The plants used in this part of the
experiment are primarily from the College of Science Greenhouse, on the roof of Duncan
Hall. Six plants were used in this study. Their names and abbreviations are summarized in
the table below.
Table 1. Plants studied in Experiment A Part III.
Preparing the Crude Extract:
Select one of the plants provided by the SJSU College of Science greenhouse. The specimens
are common plants, and some may be familiar to you. In your notes, enter the scientific and
common name of the plant (if known).
Pick about ~ 3 to 5 grams of clean leaves, and wash them gently under running water; pat
them dry with paper towels. Weigh your leaves and enter the mass in your notes.
Break or tear the leaves into small pieces (
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