Lab report April 14, 2013 Abstract: In this article, we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs. Introduction:
Enzymes are highly important because of the significant role they play in living things and animals. They are a factor which allows whether or not a reaction occurs. To find out the amount of strength an enzyme has, we need to first understand our outcome. In this experiment, our products will determine the strength of our enzymes by measuring the amount of color change. We must understand the measurement of the appearing product means in the experiment. We will determine this by using a special equipment to help us understand our result better.
In this study we will see how a specific enzyme could vary based on the different types of factors that affect it. The question we are going to be addressing in this study is, will the amount of enzyme concentration affect a reaction? Materials and Methods: The materials used in the making of this experiment are as stated: Three test tubes, 10. 5mL of distilled water, 6mL of Catechol, 1. 5mL of Extract(potato), a Spectronic 20 (spectrophotometer), Wax pencil, a Test tube rack, three Parafilm, tissues to clean the Spectronic 20 and the test tubes, 3 pipets and a timer.
So we won’t confuse ourselves, we used the wax pencil to label each test tube as we placed the required amount of substance in each of them. To avoid any slight errors, we also used three different pipets for the three required materials, which are the distilled water, catechol and the extract, respectively. It’s important to take the precise measurement so there won’t be any errors in doing the experiment. It’s also important that the extract goes last due to the reaction occurring in a timed pace. Results:
According to the graph shown above, we see our lay out of the experiment being done. We can see how the materials are being distributed in each test tube according to the amount of ingredients required. This is highly crucial because a slight mistake can impact the experiment being done. Our end result is as follows: According to the experiment done today, we see that in test tube two, it show the most absorbance or color change. This shows that the enzyme’s strength was stronger than the rest, resulting into having more reaction and color change among the others.
Discussion: As stated before, the question we had was, will the amount of enzyme concentration affect a reaction? The end result was yes, the amount of enzyme concentrated inserted into each test tube did have a significant affect to the reaction by the amount of products it gave off (color strength). We used the extract (enzyme) as our independent variable because this is what we are testing. In contrast, we used the amount of absorbance as our dependent variable.
We also made sure that our control done in this experiment was the catechol. As predicted, we were able to have more enzyme reaction in one of the test tubes than the others. The knowledge we gain today was how much the amount of concentration enzyme affects the other ingredients. We see that our experiment done today can further lead into more research as in how enzymes are used to rapid an action. It’s also important because we should know the reason why and how enzymes reactions occur at a fast rate.
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