need high standard, professional scientific writer for National Science Foundation: NSF grant proposal

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 need high standard, professional scientific writer for National Science Foundation: NSF grant proposal 

Title: (potential title, feel free to make suggestions) A study of the molecule mechanism of oligo-peptides in dehydration stress in plant

II. Keywords and Abstracts (English)

List keywords and summarize the main points of this grant proposal.

1. Abstract in English (maximum 500 words).

(In order to help you-

1. Here is the information for you about the what is the significant biological and scientific questions, direction, in this proposal, and how will we plan to design experiments to address the specific questions in this proposal.

2. the proposal should start with abstract, introduction, significants, specific aims, and how do you plan to address them, and what results do you expect to get? And future plan.

Such as

Here is the summary for this research current and future plan.

1. Duo to the global warming, how to prevent water lost is important for planta

2. Phytohormone, ABA plays a key role under dehydration stress in plans.

3. Peptides function as nutrient, also as signal molecule.

4. oligo-peptide transporters n Arabidopsis is responsible for oligo-peptide as well as ABA transport.

5. Addition oligopeptide can cause stromata close and prevent water lost under dehydration stress.

6. the mutants of oligo-peptide transporters, PTRs, shown participated in regulation of dehydration stress in Arabidopsis.

7. the aim for this study is to understand the underlying mechanisms of PTRs mediated oligo-peptide transport and signaling under dehydration stress.

8. Strategies & methods: by a mutant screening for dehydration sensitivity, under oligo peptide as solo nitrogen condition, we will identify the key genes which participate in oligo-peptide and dehydration stress.

9. Protein- protein interaction, such as Yeast-two hybrid, BiFC, in vitro and in vivo will further be used for understanding the relationship of interactions.

10. We will measure the fresh weight, water content photosynthesis activity, recover rate, as well as yields, to confirm and understand the regulation mechanisms.

11. This proposal is trying to study the functions of oligo-peptide in dehydration stress in Arabidopsis and try to identify the key genes regulated the whole pathway.

This should a four to five years grant proposal. Each year has specific plan.

III. Contents of Grant Proposal (written in Chinese or English):

1. Research project’s background: Describe in detail the intended issues to be investigated or resolved, research originality, significance, anticipating impact, status of domestic or foreign research related to this project, and bibliography of major reference materials, etc. For continued projects, describe the progress of the research from the prior year.

1. Methods, procedures, and progress: Describe for each year–

(1)Research principles, methods, and the innovation of research methods.

(2)Anticipated problems and means of resolution.

(3)Support for use of major instrumentation.

(4)If applying for travel expenses for Mainland China and international destinations, describe necessity and anticipated results.

2. Anticipated results and achievements: Describe for each year–

(1)Anticipated research tasks to be carried out.

(2)Potential training to be gained by personnel participating in this project.

(3)Anticipated research outcomes (Ex: practical applications Journal articles, conference papers, monographs, technical reports, patents or technology transfer in quality or in quantity).

(4)Potential contribution to academics, national developments, or other applications.

3. Integrated research project: State overall relevance with other sub-projects for each point listed above.

I send you some references of paper about dehydration stress, oligo-peptide, transporters of oligo peptide, which you can try to build up the story alone with the aims.

The structure of Proposal should as follow:

1. Summary (should including introduction, questions to address, and how to address them, expedition of results after finished this proposal)

2. Main body:

1) introduction: i) should describe and give enough information about why study in water loss is important to plant and human, ii) what are the important roles of oligo peptides in planta, and what do we know so far about the relationship between peptides and water loss? iii) PTRs is the major oligo-peptide transporters in plants, and they also can transport phytohormones, iv) what is unkown so far and what is the specific question you want to address and v) how do you plan to address them with experiments?

Our preliminary data:

1) Oliog peptide treated plants will triggered stromata closed compared with 1/2 MS medium, 2) photosynthesis activity is higher in the plants, which treated with oligo peptide, 3) Water loss is less in the plant who treated with oligo-peptide. PTR mutants shown hypersensitivitive or resistant phenotypes to water loss stress condition.

Regarding to our preliminary data, we will try to address that why oligo peptide can help plant overcome water loss, by what kinds of molecule mechanisms in plant? How does PTR transporters involved in this pathway? And what are the key regulators to regulate PTR to prevent from water loss.

The plan in this proposal is 1) test different oligo peptides and various concentrations of peptides for the specificity and affinity in water loss 2) to identified the key regulators by phenotype screening from mutant lines. 3) analysis of water and oilogo-peptide as well as ABA content in wild type and mutants. 4) will perform protein-protein interaction assays via in vivo and in vitro such as, Yeast-two hybrid, BiFC, in vitro functional assay in oocytes.